kanamycin resistance造句

• Promoter-probe vector psupv4 was used to clone promoters of pseudomonas pseudoalcaligenes in escherichia coli . nine kanamycin resistant recombinants were obtained and designated as ppal-ppa9 . the ppa7, which has the highest kanamycin resistance, was chose for further characterization
  利用启动子探针型载体psupv4直接在大肠杆菌中克隆类产碱假单胞菌(pseudomonaspseudoalcaligenes)基因启动子片段，获得具有强启动子的重组子ppa7。
• The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation . transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting, through the phenotype observation of transgenic plants, the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made
  最后，融合基因被插入二元载体plbj21，通过电击法转入农杆菌gv3101，真空渗入法转入了拟南芥，转基因拟南芥通过kan抗性筛选而得到，并进一步进行了基因组pcr鉴定和western杂交的鉴定。
• Three sub cloned recombinants were gained and named as ppa7-1 ppa7-2 ppa7-3 each has different inserting fragments . all the three sub cloned recombinants have the same kanamycin resistance about 600u g / ml . the result shows that the pa7-2 fragment still has promoter activity . the absence of 0.9kb from 3 " end had no influence on the promoter activity, while the absence of 0.7kb from 5 " end led the decrease of kanamycin resistance, which indicates that this 0
  卡那霉素抗性测定发现三种次克隆重组子的抗性相似，为600gml左右，证明ppa7?2所克隆的5’端899-1120bp片段在大肠杆菌中具有启动子的功能，5’端1120bp后的片段缺失对启动子没有影响。5’端0.7kb片段缺失明显影响启动子活性，推测该片段可能对启动子具有增强的作用。
• Three sub cloned recombinants were gained and named as ppa7-1 ppa7-2 ppa7-3 each has different inserting fragments . all the three sub cloned recombinants have the same kanamycin resistance about 600u g / ml . the result shows that the pa7-2 fragment still has promoter activity . the absence of 0.9kb from 3 " end had no influence on the promoter activity, while the absence of 0.7kb from 5 " end led the decrease of kanamycin resistance, which indicates that this 0
  卡那霉素抗性测定发现三种次克隆重组子的抗性相似，为600gml左右，证明ppa7?2所克隆的5’端899-1120bp片段在大肠杆菌中具有启动子的功能，5’端1120bp后的片段缺失对启动子没有影响。5’端0.7kb片段缺失明显影响启动子活性，推测该片段可能对启动子具有增强的作用。
• The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment . it showed that the best explant was the big leaf, on which the transformation frequency was 22 % . by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling
  利用双元载体的根癌农杆菌lba4404菌株(agrobacteriumtumefaciens)，含质粒pyhy(目的基因及npt、gus基因)，对白桦试管苗茎段，叶柄，叶片三种外植体进行侵染，结果表明：大叶片生长势强，为转基因的最优外植体，转化率能够达到22。